Trypsin-Based Chemoenzymatic Assay for Detection of Pollutants and Safety Assessment of Food Additives

Chemoenzymatic assay systems are widely used to detect toxicants in various samples, including food and environment specimens. These methods based on the ability of types toxicant specifically inhibit/activate functions individual enzymes or enzyme systems. The present study examines possibility using proteolytic trypsin as a specific marker protease inhibitors different samples. shows that activity is not affected by heavy metals, pesticides, quinones at levels considerably greater than their maximum allowable concentrations (MACs) water bodies. At same time, IC50 value for preservative potassium sorbate (E202) 15 mg/L, which substantially lower its acceptable daily intake (ADI). quenching fluorescence presence suggests inhibition could occur due binding region adjacent active center. was immobilized starch gel ensure stability assay. Single-use reagents were prepared dry disks be stored over long periods. Their sensitivity copper (II) chloride, sorbate, chromium (III) chloride similar free trypsin.